Role of the fungus-specific flavin carrier Flc1 in antifungal resistance in the fungal pathogen Cryptococcus neoformans.
Identifieur interne : 000243 ( Main/Exploration ); précédent : 000242; suivant : 000244Role of the fungus-specific flavin carrier Flc1 in antifungal resistance in the fungal pathogen Cryptococcus neoformans.
Auteurs : Ping Zhang [République populaire de Chine] ; Chenxi Li [République populaire de Chine] ; Liang Huo [République populaire de Chine] ; Biyun Xiang [République populaire de Chine] ; Kashif Rahim [République populaire de Chine] ; Xiaoran Hao [République populaire de Chine] ; Xudong Zhu [République populaire de Chine]Source :
- Medical mycology [ 1460-2709 ] ; 2019.
Descripteurs français
- KwdFr :
- Antifongiques (pharmacologie), Biologie informatique (MeSH), Chitine (métabolisme), Cryptococcus neoformans (métabolisme), Dodécyl-sulfate de sodium (métabolisme), Humains (MeSH), Paroi cellulaire (métabolisme), Phylogenèse (MeSH), Protéines de transport membranaire (génétique), Protéines de transport membranaire (métabolisme), Protéines fongiques (génétique), Protéines fongiques (métabolisme), Rouge Congo (métabolisme), Résistance des champignons aux médicaments (MeSH), Similitude de séquences (MeSH), Techniques de knock-out de gènes (MeSH).
- MESH :
- génétique : Protéines de transport membranaire, Protéines fongiques.
- métabolisme : Chitine, Cryptococcus neoformans, Dodécyl-sulfate de sodium, Paroi cellulaire, Protéines de transport membranaire, Protéines fongiques, Rouge Congo.
- pharmacologie : Antifongiques.
- Biologie informatique, Humains, Phylogenèse, Résistance des champignons aux médicaments, Similitude de séquences, Techniques de knock-out de gènes.
English descriptors
- KwdEn :
- Antifungal Agents (pharmacology), Cell Wall (metabolism), Chitin (metabolism), Computational Biology (MeSH), Congo Red (metabolism), Cryptococcus neoformans (metabolism), Drug Resistance, Fungal (MeSH), Fungal Proteins (genetics), Fungal Proteins (metabolism), Gene Knockout Techniques (MeSH), Humans (MeSH), Membrane Transport Proteins (genetics), Membrane Transport Proteins (metabolism), Phylogeny (MeSH), Sequence Homology (MeSH), Sodium Dodecyl Sulfate (metabolism).
- MESH :
- chemical , genetics : Fungal Proteins, Membrane Transport Proteins.
- chemical , metabolism : Chitin, Congo Red, Fungal Proteins, Membrane Transport Proteins, Sodium Dodecyl Sulfate.
- chemical , pharmacology : Antifungal Agents.
- metabolism : Cell Wall, Cryptococcus neoformans.
- Computational Biology, Drug Resistance, Fungal, Gene Knockout Techniques, Humans, Phylogeny, Sequence Homology.
Abstract
FLC family, a conserved fungus-specific family of integral membrane proteins, has been demonstrated to play important roles in flavin transport, growth, and virulence in several fungi but not yet in Cryptococcus neoformans. In this study, we have identified the single homologue of flavin adenine dinucleotide transporter in the opportunistic pathogen C. neoformans. The computational and phylogenetic analysis confirmed the fungal specificity of cryptococcal Flc1 protein, thus providing a promising drug target for clinical treatment of cryptococcosis. Disruption of FLC1 conferred sensitivity to 1% Congo red and 0.02% SDS, as well as leading to impaired chitin distribution in cell wall as observed with Calcofluor White staining, which collectively indicated the roles of FLC1 in maintenance of cell wall integrity. Further investigations revealed the defects of flc1Δ mutant in resistance to poor nutrition and elevated temperatures, and the ability to undergo invasive growth under nutrient-depleted conditions was reduced as well in flc1Δ mutant, suggesting the roles of Flc1 in response to environmental stresses. More importantly, our results showed that flc1Δ mutant exhibited severe susceptibility to antifungal aminoglycosides (hygromycin B and geneticin) and amphotericin B, but developed multidrug resistance to flucytosine and rapamycin, which provided great hints for therapeutic failure of cryptococcosis in clinic with the standard combination therapy. Finally, typical virulence factors including melanin biosynthesis and capsule formation in flc1Δ mutant were reduced as well, indicating the possible involvement of Flc1 in virulence.
DOI: 10.1093/mmy/myy050
PubMed: 30010978
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Role of the fungus-specific flavin carrier Flc1 in antifungal resistance in the fungal pathogen Cryptococcus neoformans.</title><author><name sortKey="Zhang, Ping" sort="Zhang, Ping" uniqKey="Zhang P" first="Ping" last="Zhang">Ping Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Li, Chenxi" sort="Li, Chenxi" uniqKey="Li C" first="Chenxi" last="Li">Chenxi Li</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Huo, Liang" sort="Huo, Liang" uniqKey="Huo L" first="Liang" last="Huo">Liang Huo</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Xiang, Biyun" sort="Xiang, Biyun" uniqKey="Xiang B" first="Biyun" last="Xiang">Biyun Xiang</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Rahim, Kashif" sort="Rahim, Kashif" uniqKey="Rahim K" first="Kashif" last="Rahim">Kashif Rahim</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Hao, Xiaoran" sort="Hao, Xiaoran" uniqKey="Hao X" first="Xiaoran" last="Hao">Xiaoran Hao</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhu, Xudong" sort="Zhu, Xudong" uniqKey="Zhu X" first="Xudong" last="Zhu">Xudong Zhu</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:30010978</idno><idno type="pmid">30010978</idno><idno type="doi">10.1093/mmy/myy050</idno><idno type="wicri:Area/Main/Corpus">000499</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000499</idno><idno type="wicri:Area/Main/Curation">000499</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000499</idno><idno type="wicri:Area/Main/Exploration">000499</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Role of the fungus-specific flavin carrier Flc1 in antifungal resistance in the fungal pathogen Cryptococcus neoformans.</title><author><name sortKey="Zhang, Ping" sort="Zhang, Ping" uniqKey="Zhang P" first="Ping" last="Zhang">Ping Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Li, Chenxi" sort="Li, Chenxi" uniqKey="Li C" first="Chenxi" last="Li">Chenxi Li</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Huo, Liang" sort="Huo, Liang" uniqKey="Huo L" first="Liang" last="Huo">Liang Huo</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Xiang, Biyun" sort="Xiang, Biyun" uniqKey="Xiang B" first="Biyun" last="Xiang">Biyun Xiang</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Rahim, Kashif" sort="Rahim, Kashif" uniqKey="Rahim K" first="Kashif" last="Rahim">Kashif Rahim</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Hao, Xiaoran" sort="Hao, Xiaoran" uniqKey="Hao X" first="Xiaoran" last="Hao">Xiaoran Hao</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhu, Xudong" sort="Zhu, Xudong" uniqKey="Zhu X" first="Xudong" last="Zhu">Xudong Zhu</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">Medical mycology</title><idno type="eISSN">1460-2709</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antifungal Agents (pharmacology)</term><term>Cell Wall (metabolism)</term><term>Chitin (metabolism)</term><term>Computational Biology (MeSH)</term><term>Congo Red (metabolism)</term><term>Cryptococcus neoformans (metabolism)</term><term>Drug Resistance, Fungal (MeSH)</term><term>Fungal Proteins (genetics)</term><term>Fungal Proteins (metabolism)</term><term>Gene Knockout Techniques (MeSH)</term><term>Humans (MeSH)</term><term>Membrane Transport Proteins (genetics)</term><term>Membrane Transport Proteins (metabolism)</term><term>Phylogeny (MeSH)</term><term>Sequence Homology (MeSH)</term><term>Sodium Dodecyl Sulfate (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Antifongiques (pharmacologie)</term><term>Biologie informatique (MeSH)</term><term>Chitine (métabolisme)</term><term>Cryptococcus neoformans (métabolisme)</term><term>Dodécyl-sulfate de sodium (métabolisme)</term><term>Humains (MeSH)</term><term>Paroi cellulaire (métabolisme)</term><term>Phylogenèse (MeSH)</term><term>Protéines de transport membranaire (génétique)</term><term>Protéines de transport membranaire (métabolisme)</term><term>Protéines fongiques (génétique)</term><term>Protéines fongiques (métabolisme)</term><term>Rouge Congo (métabolisme)</term><term>Résistance des champignons aux médicaments (MeSH)</term><term>Similitude de séquences (MeSH)</term><term>Techniques de knock-out de gènes (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Fungal Proteins</term><term>Membrane Transport Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Chitin</term><term>Congo Red</term><term>Fungal Proteins</term><term>Membrane Transport Proteins</term><term>Sodium Dodecyl Sulfate</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antifungal Agents</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines de transport membranaire</term><term>Protéines fongiques</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cell Wall</term><term>Cryptococcus neoformans</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Chitine</term><term>Cryptococcus neoformans</term><term>Dodécyl-sulfate de sodium</term><term>Paroi cellulaire</term><term>Protéines de transport membranaire</term><term>Protéines fongiques</term><term>Rouge Congo</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antifongiques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Computational Biology</term><term>Drug Resistance, Fungal</term><term>Gene Knockout Techniques</term><term>Humans</term><term>Phylogeny</term><term>Sequence Homology</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biologie informatique</term><term>Humains</term><term>Phylogenèse</term><term>Résistance des champignons aux médicaments</term><term>Similitude de séquences</term><term>Techniques de knock-out de gènes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">FLC family, a conserved fungus-specific family of integral membrane proteins, has been demonstrated to play important roles in flavin transport, growth, and virulence in several fungi but not yet in Cryptococcus neoformans. In this study, we have identified the single homologue of flavin adenine dinucleotide transporter in the opportunistic pathogen C. neoformans. The computational and phylogenetic analysis confirmed the fungal specificity of cryptococcal Flc1 protein, thus providing a promising drug target for clinical treatment of cryptococcosis. Disruption of FLC1 conferred sensitivity to 1% Congo red and 0.02% SDS, as well as leading to impaired chitin distribution in cell wall as observed with Calcofluor White staining, which collectively indicated the roles of FLC1 in maintenance of cell wall integrity. Further investigations revealed the defects of flc1Δ mutant in resistance to poor nutrition and elevated temperatures, and the ability to undergo invasive growth under nutrient-depleted conditions was reduced as well in flc1Δ mutant, suggesting the roles of Flc1 in response to environmental stresses. More importantly, our results showed that flc1Δ mutant exhibited severe susceptibility to antifungal aminoglycosides (hygromycin B and geneticin) and amphotericin B, but developed multidrug resistance to flucytosine and rapamycin, which provided great hints for therapeutic failure of cryptococcosis in clinic with the standard combination therapy. Finally, typical virulence factors including melanin biosynthesis and capsule formation in flc1Δ mutant were reduced as well, indicating the possible involvement of Flc1 in virulence.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">30010978</PMID><DateCompleted><Year>2019</Year><Month>04</Month><Day>29</Day></DateCompleted><DateRevised><Year>2019</Year><Month>04</Month><Day>29</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1460-2709</ISSN><JournalIssue CitedMedium="Internet"><Volume>57</Volume><Issue>4</Issue><PubDate><Year>2019</Year><Month>Jun</Month><Day>01</Day></PubDate></JournalIssue><Title>Medical mycology</Title><ISOAbbreviation>Med Mycol</ISOAbbreviation></Journal><ArticleTitle>Role of the fungus-specific flavin carrier Flc1 in antifungal resistance in the fungal pathogen Cryptococcus neoformans.</ArticleTitle><Pagination><MedlinePgn>468-477</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/mmy/myy050</ELocationID><Abstract><AbstractText>FLC family, a conserved fungus-specific family of integral membrane proteins, has been demonstrated to play important roles in flavin transport, growth, and virulence in several fungi but not yet in Cryptococcus neoformans. In this study, we have identified the single homologue of flavin adenine dinucleotide transporter in the opportunistic pathogen C. neoformans. The computational and phylogenetic analysis confirmed the fungal specificity of cryptococcal Flc1 protein, thus providing a promising drug target for clinical treatment of cryptococcosis. Disruption of FLC1 conferred sensitivity to 1% Congo red and 0.02% SDS, as well as leading to impaired chitin distribution in cell wall as observed with Calcofluor White staining, which collectively indicated the roles of FLC1 in maintenance of cell wall integrity. Further investigations revealed the defects of flc1Δ mutant in resistance to poor nutrition and elevated temperatures, and the ability to undergo invasive growth under nutrient-depleted conditions was reduced as well in flc1Δ mutant, suggesting the roles of Flc1 in response to environmental stresses. More importantly, our results showed that flc1Δ mutant exhibited severe susceptibility to antifungal aminoglycosides (hygromycin B and geneticin) and amphotericin B, but developed multidrug resistance to flucytosine and rapamycin, which provided great hints for therapeutic failure of cryptococcosis in clinic with the standard combination therapy. Finally, typical virulence factors including melanin biosynthesis and capsule formation in flc1Δ mutant were reduced as well, indicating the possible involvement of Flc1 in virulence.</AbstractText><CopyrightInformation>© The Author(s) 2018. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Ping</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Chenxi</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Huo</LastName><ForeName>Liang</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xiang</LastName><ForeName>Biyun</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rahim</LastName><ForeName>Kashif</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hao</LastName><ForeName>Xiaoran</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Xudong</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology, Institute of Biochemistry and Biotechnology, College of Life Sciences, Beijing Normal University (CLS-BNU), Beijing 100875, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Med Mycol</MedlineTA><NlmUniqueID>9815835</NlmUniqueID><ISSNLinking>1369-3786</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000935">Antifungal Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D026901">Membrane Transport Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>1398-61-4</RegistryNumber><NameOfSubstance UI="D002686">Chitin</NameOfSubstance></Chemical><Chemical><RegistryNumber>368GB5141J</RegistryNumber><NameOfSubstance UI="D012967">Sodium Dodecyl Sulfate</NameOfSubstance></Chemical><Chemical><RegistryNumber>3U05FHG59S</RegistryNumber><NameOfSubstance UI="D003224">Congo Red</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000935" MajorTopicYN="N">Antifungal Agents</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002686" MajorTopicYN="N">Chitin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019295" MajorTopicYN="N">Computational Biology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003224" MajorTopicYN="N">Congo Red</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003455" MajorTopicYN="N">Cryptococcus neoformans</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D025141" MajorTopicYN="Y">Drug Resistance, Fungal</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D055786" MajorTopicYN="N">Gene Knockout Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D026901" MajorTopicYN="N">Membrane Transport Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017385" MajorTopicYN="N">Sequence Homology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012967" MajorTopicYN="N">Sodium Dodecyl Sulfate</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Cryptococcus neoformans
</Keyword><Keyword MajorTopicYN="N">antifungal resistance</Keyword><Keyword MajorTopicYN="N">flavin carrier</Keyword><Keyword MajorTopicYN="N">fungus-specific transporter</Keyword><Keyword MajorTopicYN="N">virulence</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>03</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>04</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>07</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>7</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>4</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>7</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30010978</ArticleId><ArticleId IdType="pii">5054077</ArticleId><ArticleId IdType="doi">10.1093/mmy/myy050</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Zhang, Ping" sort="Zhang, Ping" uniqKey="Zhang P" first="Ping" last="Zhang">Ping Zhang</name></noRegion><name sortKey="Hao, Xiaoran" sort="Hao, Xiaoran" uniqKey="Hao X" first="Xiaoran" last="Hao">Xiaoran Hao</name><name sortKey="Huo, Liang" sort="Huo, Liang" uniqKey="Huo L" first="Liang" last="Huo">Liang Huo</name><name sortKey="Li, Chenxi" sort="Li, Chenxi" uniqKey="Li C" first="Chenxi" last="Li">Chenxi Li</name><name sortKey="Rahim, Kashif" sort="Rahim, Kashif" uniqKey="Rahim K" first="Kashif" last="Rahim">Kashif Rahim</name><name sortKey="Xiang, Biyun" sort="Xiang, Biyun" uniqKey="Xiang B" first="Biyun" last="Xiang">Biyun Xiang</name><name sortKey="Zhu, Xudong" sort="Zhu, Xudong" uniqKey="Zhu X" first="Xudong" last="Zhu">Xudong Zhu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/RapamycinFungusV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000243 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000243 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= RapamycinFungusV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:30010978
|texte= Role of the fungus-specific flavin carrier Flc1 in antifungal resistance in the fungal pathogen Cryptococcus neoformans.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:30010978" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a RapamycinFungusV1
| This area was generated with Dilib version V0.6.38. |  |